Rna Library Prep Protocol

The volume and concentration of the rna and also the mgcl 2 is critically important for the success of fragmentation.

Rna library prep protocol.

The small rna seq library prep kit provides a protocol for generating small rna libraries for illumina sequencing directly from total rna or enriched small rna. Multiplexing of up to 96 samples multiplexing of up to 96 samples is possible with complimentary i7 indexes provided in the kit. This total rna seq library prep kit s protocol is fragmentation free and works with up to 2 ug of undepleted total rna down to 50 ng of undepleted total rna or as little as 5 ng of ribosome depleted rna samples. Library preparation for the illumina sequencing platform requires inputs of a defined length therefore fragmentation of dna or the use of cdna prepared from rna is the starting point.

Illumina library prep protocols accommodate a range of throughput needs from lower throughput protocols for small labs to fully automated library preparation workstations for large laboratories or genome centers. Illumina stranded mrna prep a simple scalable cost effective rapid single day solution for analyzing the coding transcriptome leveraging as little as 25 ng input of standard non degraded rna. New england biolabs offers a series of highly pure reagents that facilitate library preparation of total rna single cell low input rna and small rna for next generation sequencing. Rna and small rna libraries are prepared using different workflows that are tailored to the downstream sequencing platform that they will be used with.

The nebnext ultra rna library prep kit for illumina contains reagents for preparation of non directional rna libraries for illumina sequencing. Please note that adaptors primers rrna depletion reagents and poly a mrna isolation reagents are not included in the kit and are available separately. Please see the primers process at the bottom of the protocol for a better description of primers. Pre mixed reagents are ready to use at every step eliminating the need for manual master mix preparation.

A high performing fast and integrated workflow for sensitive applications such as human whole genome sequencing. Iv document 15004197v02 document date descriptionofchange part 15004197rev e february 2013 movedworkflowdiagramtostartofprotocol.